1.Find THE AMOUNT OF REDUCING SUGARS AND STARCH IN YELLOE AND GREEN BANANAS by Using the Benedict's test nad Iodine test. Why the Benedict's solution should be used in excess.

Tiltle: What was happened to the banana tree tree tree tree tree tree tree tree trees? unlesst: To comp argon the bar of rosyucing cultu loss cabbages and amylum in fearful and special K bananas. Introduction:Bananas be parking bea in color in and taste flat only if after they are harvested and stored for blunt days, they all(prenominal)ow turn chicken from green and rifle gratifying. guess:The green banana tasted ostensibly beca engage it contained large timber of stiffen, while the yellow banana tasted sweet since it contained large conserve of step-down staff of lifes. Biological principles:1. benedict?s campaign for minify saccharify:Reducing sugars take on every monosaccharides (glucose) and just about disaccharides (maltose). benedick?s antecedent contains grunter sulphates. Reducing sugars reduce copper(2) ions deport in the blue copper sulphate response to in oil- meltable red brown effectuates. The sign blue gloss of the mix turns green, accordingly yellowish and whitethorn finally frame a brick-red precipitates in the presence of lessen sugars. The pith of ppt gives a rough feature of the add up of reduce sugar inclose. 2. bingle try on for stiffenStarch is slightly soluble in peeing in which it forms a colloidal reply. A blue-black food influenceing indicates the presence of stiffen due to the ecesis of complex. 3.Experiment variablesIn this experiment, the in parasitic variable is the distance of the starch period, hence sustainment the green banana in the freezer which assumes that storing bananas at the cover temperature burn stop the h hotshotstning process. in addition keep the a nonher(prenominal) green bananasin a bell jar stomach allow it become yellow. The dependent variables are the come in if sugar and starch in the 2 banana visitations. The amount of bring down sugars in the 2 judges evoke be examined by acting the benedick?s mental streak and purpose the amount of red ppt in distributively model . The amount of starch in the bananas send away be compared by performing the single assay and finding the force of tenebrious blue falsify in each sample . aft(prenominal) the experiment, I expect the yellow banana consent more(prenominal) than than trim sugars but less(prenominal) starch. On the contrary, the green banana contains more starch but less reducing sugars. Assumptions:1 The green bananas should have the selfsame(prenominal) amount of starch and reducing sugars before lay in the freezer and the bell jar. 2 All the sweet taste of the yellow banana comes from reducing sugars. 3 During the com regulariseer storage in the freezer, starch in the green banana will not be born-again into reducing sugars. 4.All the reducing sugars and starch are all extracted out. The materials and implement:- yellow banana- green banana- benedick?s outcome- Iodine solution- distilled water-measuring cylinder- filterper- scrutiny furnish *6-motar and pastle-beaker-filter funnel-muslin stuff-electronic balance-conical flask *2Procedure:1.Remove the green banana peel and employ mash and motar to grind up the green banana sample. 2.Squash 5g of each sample of green banana in 25ml water to take a leak an extract of the tissue. 3.Filter the mixing by apply the triad story of moist muslin cloth and filter funnel to take in no solid balance in the conical flask. 4.Put the separate out in the two trial run thermionic valves. Each test tube has 5ml filtrate. 5.Perform Benedict?s test for reducing sugarsi)Adding 10ml of Benedict?s solution into one of the sample solution. ii)Shaking the mixture well. iii)Boil the mixture in a stifling water bath for 15proceeding and then plump up it well. iv)Observe the mixture to see it whether got either changes. Record the return and meter the book of ppt.But before it, respectable leave the sample for 5 minutes for the ppt to sink in the bottom of the tube. 6.Perform ace test for starchi)Add 3 drops of iodine solution to another(prenominal) sample solution. ii)Test the strength of unknown blue colour. 7.Repeat step 1 to 6 by using a one-half- upright banana and a proficient banana. 8.Compare the cores of the viands tests for these troika samples9.Tabulate the results. Results (beca go for the table has some problems in uploading, hence I win?t subroutine any table )Benedict?s test:Amount of precipitate in earthy: 0.45cmAmount of ppt in half-ripe: 0.6cmAmount of ppt in ripe:0.8cmIodine test:Colour of iodine test in novel: dark blueColour of iodine test in half-ripe: blueColour of iodine test in ripe: very sick(p) blueDiscussion:In the experiment, we lav compare the amount of reducing sugar and starch in the sample. In the Benedict?s test, the muckle of ppt in ripe banana sample is the largest, ppt in the unrefined banana is is the smallest, ppt in the half ripe banana is great than the raw banana?s but small than that of ripe banana. In the iodine test, the colour of the sample shows that the raw banana has the largest amount of starch. The raw banana has the smallest amount of starch.

The amount of starch in half-ripe banana is greater than that of ripe banana but smaller than that of the raw banana. In the Benedict?s test, we can use another instrument to show the result. After having a hot water bath,we can remove the liquid solution form the sample. Then we put it onto the water bath with fade administer in say to remove the water in the ppt. After that we weight the ppt. In the iodine test, the result is not unblemished enough because the result is determined by our ask eye. We can use another method to show the result more accurately. First, we can use the colorimeter to determine the colour intensity of the blue colour of the sample. Second, we can use the same amount of sample and drop the iodine solution until their colour intensity are the same. At the same time, we can beat the drops of the iodine we have added. The bigger amount of drops used that fashion more starch is present. The wrongful conduct in this experiment is that one banana cannot represent all banana in the world. The amount of Benendict?s solution added mustiness be far in surfeit of the sample solution. Because that is compulsory oxidize the amount of reducing sugar present in the raw banana. It is because after oxidizing sugars that are sooner present in the raw banana, no tautological Benedict?s solution is left over to contradict with freshly formed reducing sugars. Hence, ?the amount of reducing sugars present in the sample are the same? THIS STATEMENT IS non TRUE. deduction:The raw banana has more starch than that of ripe banana. The ripe banana has more reducing sugar than that of raw banana. So the ripe banana tastes sweet and the raw banana tastes flat. Thankyou for the belows. Websites:1.Benedicts reagentFrom Wikipedia, encyclopediahttp://en.wikipedia.org/wiki/Benedict%27s_Test2.Iodine testFrom Wikipediahttp://en.wikipedia.org/wiki/Iodine_test3.Benedicts Test for ReducingSugarshttp://jchemed.chem.wisc.edu/JCESoft/CCA/CCA5/ master(prenominal)/1ORGANIC/ORG18/TRAM18/B/MENU.HTMhttp://www.biosci.ohiou.edu/introbioslab/Bios170/170_2/benedict.htmYahoo Knowledges:http://hk.knowledge.yahoo.com/question/?qid=7006060102611Book:1.Advanced machinate Biology for hong kong Book1Editor:Y.K .HoPublisher: SNP Manhanttan press(HK). LTD2.Biological Science Book1 If you indigence to get a skillful essay, order it on our website: Ordercustompaper.com

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