Monoclonal antibodies

The production of monoclonal antibodies, using the hybridoma methodMonoclonal antibodies atomic number 18 obtained from clones of single B cells. Unfortunately, B cells bequeath not get into in gardening and this problem has to be got round by fusing them withmalignant B myeloma cells i.e. malignant B cells. Myeloma cells will continue to wrench and single out indefinitely, though they do not prepare antibodies. The f rehearsed cellsproduced from myeloma cells with B cells be known as hybridomas. The hybridoma cells will also continue to grow and depart (given suitable and adequate to(predicate) nutrients) andthey do exude antibodies. The antibodies that they secrete argon the specific antibodies that were produced by the original clone of B cells.

The production of monoclonal antibodies involves the pursual stages :? A mo affair is injected with antigen for which theantibodies are required? An immune solvent takes place and the mouse plasmacells set off to light upon caput the antibody? Plasma cells are extracted from the mouse? The plasma cells are f utilise with B cell myeloma cells? The resulting hybridoma cells are separated individu eachyand all in allowed to grow, divide and produce antibodies? Some antibodies are removed and tested with the relevantantigen, to make sure they are the correct monclonals? Those hybridoma cells which are producing the requiredantibodies are stopping point in a large fermenter? The monoclonal antibodies are harvested and purifiedThe use of monoclonal antibodies in the diagnosis and treatment of disease and pregnancy testingDiagnosis ? because the monoclonal antibodies produced f! rom a clone of B cells are all identical, they can be utilize to identify macromolecules with a tiny high degree ofspecificity. For example, they are now routinely used for the following:? Blood type before transfusions? Tissue typewrite before transplants? Identification of pathogens ? using monoclonals, it is now possible to nock between different strains of certain pathogens, which would, otherwise, be every surd? Identification... If you want to get a full essay, order it on our website: OrderCustomPaper.com

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